ActiTOX: active organotypic models for nanoparticle toxicological screening

To increase the relevance of in-vitro toxicological studies the project will:

·         Develop 3D biomimetic scaffolds with chemical and mechanical properties optimized for a particular tissue.

·         Develop active and cell-stimulating scaffolds that provide the chemical signalling during the cell differentiation and growth.

·         Customizing the bioactive molecules release from scaffolds and establish the protocols for regulated differentiation of induced pluripotent stem cells to desired cell lines.

·         Develop a bi-organ bioreactor platform with two flow circuits: for particle exposure, and for simulation of vascular flow.

·         Reduce the overall cost of the toxicological screening by lowering the consumption of active molecules and combining the absorption and metabolism testing into one device.